Test probes idiki-stranded single-stranded DNA kana RNA zvidimbu (inenge 20 kusvika 500 bp) inoshandiswa kuona complementary nucleic acid sequences.DNA ine tambo mbiri inodzorwa nekudziya kuti ive imwe-stranded, yobva yanyorwa neradioisotopes (kazhinji phosphorus-32), madhayi efluorescent, kana ma enzymes (akadai sehorseradish peroxidase) kuita probes.Phosphorus-32 inowanzobatanidzwa muboka re phosphate reimwe ye mana nucleotide anoumba DNA, uye madhayi efluorescent uye ma enzymes akabatanidzwa zvakabatana kune nucleic acid sequences.
Ikozvino muyedzo yekumisikidza, iyo yekuyedza probe inoshandiswa sesvikiro, probe inoiswa mucasing, musoro weprobe unobata chinhu chinofanirwa kuongororwa, uye waya inotungamira yecasing kune imwe magumo inoitisa chiratidzo, uye iyo chiratidzo chinogamuchirwa chiri mu tester.Semuyenzaniso, resistor inoshandisa yazvino sosi kuverenga kudonha kwevoltage pamhiri peprobe, uye capacitor inoshandisa inogara voltage sosi kuverenga kutsetseka kwenguva yekuchaja pama frequency akasiyana.Iyo probe ine hunhu hwekuenderera mberi kwekushandisa kana ichinge yaitwa mugadziriso, uye mutengo hauna kukwirira.Akashandiswa zvakanyanya munharaunda yekuyedza.
Kune akawanda marudzi ebvunzo probe misoro mhando.Chikonzero chikuru ndechekuti mapiidhi ebvunzo akasiyana anoda mhando dzemusoro dzakasiyana.Semuyenzaniso, tsoka dzekunyudza dzinoshandisa mhando dzemisoro dzakawanda, mapoinzi ebvunzo anoshandisa misoro yakanongedza, yakatenderera kana kuti yakati sandara, uye mapini eIC anoshandisa maruva anotumbuka.Chimiro chemusoro, nezvimwe zvose zvinosarudzwa nevashandi vane ruzivo rwekugadzira maererano nezvikamu paPCB yapera.
Nanometering ingori kusimbaradza kwezvinhu zveprobe, uye hapana chinokanganisa pane bvunzo gadziriso.Kana muchifarira, kukanganiswa kwechiyedzo kunofanira kunge kuri chikamu chemudziyo wekuyedza, sekuonekwa kweAOI.
Nguva yekutumira: May-19-2022