Umhlinzeki Wezinto Zokwelashwa Ochwepheshe

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Uyini umgomo wophenyo

Ama-probe okuhlola ama-DNA anomucu owodwa noma izingcezu ze-RNA (cishe u-20 kuya ku-500 bp) ezisetshenziselwa ukuthola ukulandelana kwe-nucleic acid.I-DNA enemicu emibili ishintshwa ngokushisisa ukuze ibe umucu owodwa, bese ibhalwa ngama-radioisotopes (imvamisa i-phosphorus-32), odayi be-fluorescent, noma ama-enzyme (afana ne-horseradish peroxidase) ukuze abe ama-probe.I-Phosphorus-32 ivamise ukufakwa eqenjini le-phosphate yeyodwa yama-nucleotide amane akha i-DNA, futhi odayi be-fluorescent nama-enzyme axhumene ngokuhlanganyela nokulandelana kwe-nucleic acid.

IMG_4666-300x200 Ukhozi FM-053-300x300

Manje endaweni yokuhlola, i-probe yokuhlola isetshenziswa njengendawo ephakathi, i-probe ifakwa ku-casing, ikhanda lophenyo lithinta into ezohlolwa, futhi ucingo oluholayo lwebhokisi ngakolunye uhlangothi luqhuba isignali, futhi isignali eyamukelwe ikumhloli.Isibonelo, i-resistor isebenzisa umthombo wamanje ukubala ukwehla kwe-voltage kuyo yonke i-probe, futhi i-capacitor isebenzisa umthombo we-voltage ongashintshi ukubala umthamo wesikhathi sokushaja kumafrikhwensi ahlukene.I-probe inezici zokusetshenziswa okuqhubekayo uma yenziwa into ethile, futhi izindleko aziphezulu.Usetshenziswe kakhulu emphakathini wokuhlola.

Kunezinhlobo eziningi zezinhlobo zekhanda le-probe yokuhlola.Isizathu esiyinhloko ukuthi amaphuzu okuhlola ahlukene adinga izinhlobo ezahlukene zamakhanda.Isibonelo, izinyawo zediphu zisebenzisa izinhlobo zamakhanda anezinzipho eziningi, amaphuzu ephedi yokuhlola asebenzisa amakhanda acijile, ayindilinga noma ayisicaba, kanti izikhonkwane ze-IC zisebenzisa i-plum blossom.Ukuma kwekhanda, njll. konke kukhethwe izisebenzi zokukhiqiza ezinolwazi ngokwezingxenye ze-PCB eqediwe.

I-Nanometering iwukuqiniswa kwezinto ze-probe kuphela, futhi awukho umthelela endaweni yokuhlola.Uma unentshisekelo, umthelela endaweni yokuhlola kufanele ibe yingxenye yethuluzi lokuhlola, njengokutholwa kwe-AOI.


Isikhathi sokuthumela: May-19-2022